The Technology - Isolation Method

Aegle’s Extracellular Vesicle Isolation Method

Aegle has developed and patented a process for the isolation and purification of extracellular vesicles (including exosomes) that results in high quality, therapeutic grade extracellular vesicles with ideal morphology and full functionality. Established methods to isolate extracellular vesicles typically utilize ultracentrifugation, immune absorption and co-precipitation methods. These techniques utilize physical and chemical processes that can alter or damage extracellular vesicles, making them unsuitable for therapeutic use and even harmful.

Aegle’s process is GMP compliant. Our patented process involves the removal of cellular debris and concentration of the extracellular vesicles. It does not require sophisticated equipment. Aegle’s method allows for large-scale collection and purification of extracellular vesicles from cell culture supernatants and biological fluids at a low cost.

Aegle’s purification method isolates a very broad size range of extracellular vesicles and produces a higher yield of extracellular vesicles per sample. Nanosight analysis supports that Aegle’s method yields a significant improvement to what has been published by conventional procedures.

Our advanced extracellular vesicle isolation method offers a unique combination of benefits including low cost, GMP compliance, scalability, potency and consistency of quality.

Photo Above: Electron micrographs of extracellular vesicles derived from medium conditioned using human BM-stem cells isolated by the ultracentrifuge method (top) and isolated according to Aegle’s isolation methods (bottom) at the magnification shown in the panels. Utracentrifuged extracellular vesicle are less abundant.